Solutions for upstream bioprocessing in Pharma and Industrial Biotechnology


Applikon Biotechnology is a world leader in developing and supplying advanced bioreactor systems for the production of vaccines, antibodies, tissues, bio-pharmaceuticals, nutraceuticals and biosimilars in the Pharmaceutical industry, as well as enzymes, food, feed, bio-fuel, bio-chemicals and bio-plastics for Industrial Biotechnology. We can guide you through the whole upstream process, from initial screening up to full-scale production, using the same platform. 

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About Applikon Biotechnology

Applikon Biotechnology proudly promotes an improved Quality of Life. As a world leader in the development and supply of advanced bioreactor systems, Applikon passionately contributes to the Life Sciences. Our goal is to continuously introduce new technologies to the market, covering the spectrum from laboratory scale via pilot plant to production scale. In addition to advanced cultivation systems, our core business also comprises Process Control and Process Information Management systems. Large investments in R&D and in-house development and production enable us to be flexible and efficient when delivering the best solution for each individual customer.

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AppliFlex ST

Disposable bioreactors Designed on Demand

  • Fully customizable to meet your specific bioprocessing needs
  • Install any number of systems to fit your workspace
  • Cultivate anywhere; no laminar flowhood, water supply or drain needed
  • Several perfusion options for continuous bioprocessing

The AppliFlex ST is a fully customizable stirred tank single-use bioreactor. By using 3D printing technology, we can create any head plate configuration that is optimal for your process. With this single-use system there is no risk of cross contamination between runs. The pre-sterilized, ready-to-use bioreactor makes your life in the lab a lot easier. No more assembling and sterilization before you can start your culture. No more cleaning after the culture is finished. Save time and costs, execute more runs, and reduce your time-to-market.


  • Integrates with software automationand automated sampling tools
  • Interchangeable with multi-use systems
  • Removable topplate
  • Shelf life 2 years
  • Up to 0.5 barg
  • Pre-sterile and ready-to-go


  • Microbial and Cell culture
  • Regenerative medicines & Stem cell cultures
  • Batch, Fed-Batch, Perfusion and Continuous cultivation
  • Screening studies
  • Media optimization
  • Process optimization

The AppliFlex ST differentiates from other single-use bioreactors by being a fully customizable stirred tank bioreactor. You can choose, or even design, the type and number of impellers, the number of liquid and gas additions and the type of sparger that are optimal for your process. No more unused ports with blind plugs, but an optimized bioreactor for your specific process development and R&D application.

The 3D printing production technology guarantees complete reproducibility between the different bioreactors guaranteeing exact and identical conditions between runs. The AppliFlex ST offers the flexibility that you need for your specific bioprocess and is available for both cell culture and microbial applications.

The AppliFlex ST can be used as single unit as well as parallel processing systems. The design of the AppliFlex ST is a scale down version of our larger laboratory scale systems guaranteeing an easy scale-up of your process from the 500 mL to the larger volume cultivations. The single-use system can be exchanged with a multi-use system whenever your process or workflow needs a different approach.

The AppliFlex ST features a fully removable topplate for operators to have easy access to the culture. Pouring into downstream processing vessels or other bioreactors has never been this easy. Optionally a closed lid is available which makes the vessel ready for transport, storage or freezing.

Water supply is not required to operate the AppliFlex ST, cooling and heating are done using electric elements, the condenser is cooled electrically as well. No expensive laminar flowhood is needed to operate the system, expect for operator handling.

The AppliFlex ST can easily be integrated in a DeltaV control installation using our V-Control software. This makes process transfer from the lab to production and from production to the lab very simple.

Glass autoclavable bioreactors

Applikon offers glass autoclavable bioreactors for cell culture applications and glass autoclavable fermentors for microbial culture applications. The systems are built according to the specific demands of a process using an extensive array of standard components. Because of the modularity and flexibility, the user can always adapt the systems to changed process demands. This results in low initial investment and low running costs. The stirred tank reactor (STR) is the most widely used bioreactor type. Glass autoclavable bioreactors and fermentors are available in jacketed and single wall versions in 2, 3, 5, 7, 15 and 20 liter total volume (20 liter only in single wall version)


  • Microbial cultures
  • Cell cultures
  • Batch
  • Fed-Batch
  • Perfusion
  • Continuous cultivation

Stainless Steel Bioreactors

The Applikon Pilot system bioreactor range is divided into a microbial range and a cell culture range of volumes. The microbial Steam-In-Place bioreactor systems come in a total volume of 20 L, 40 L, 70 L and 140 L. The cell culture Steam-In-Place bioreactor systems come in a total volume of 30 L, 60 L and 130 L. The main difference between these two lines is the aspect ratio of the bioreactors. The microbial systems have an aspect ratio of 3.0 at total volume while the cell culture systems have an aspect ratio of 1.5 at total volume. The small footprint guarantees that the minimal amount of floor space is occupied by the system. All systems are fully documented for cGMP usage and are supplied with an extensive documentation package to support validation.


A wide range of standard modules and standard packs is available to customize the systems to your specific demands. A wide range of add-on packs is available. The standard packs include pressure control, weight control, double gas inlet lines, CIP modules, biomass sensors, perfusion systems and much more.


Applikon offers a choice of ez-Control and i-Control process control systems. The i-Control can be supplied with a DeltaV controller, an Allen Bradley or a Siemens PLC. All i-Control systems support automated Sterilization and Cleaning procedures.


• Easy to clean mirror polished external finish
• Electropolished finish of all parts in contact with the culture (Ra < 0.4 µm) to allow efficient clean-in-place
• Modular design allows easy adaptation to changing process demands
• Magnetically coupled agitator for peace of mind
• cGMP compliant design and documentation simplifies validation
• Compact design reduces floor space
• Open frame construction gives easy access for maintenance and operation


• Scale-up studies
• Medium optimization
• Process optimization
• Small scale production
• Microbial and Cell culture
• Batch, Fed-Batch, Perfusion and Continuous cultivation

Applikon Biotechnology BV


Heertjeslaan 2
2629 JG Delft
The Netherlands

T. +31(0)10-208 35 55