One of many convenient PepSet™ Peptide Library designs, the Truncated Library’s key focus is to identify the shortest active peptide sequence.
Especially useful in epitope mapping, a series of short peptides decreasing in length is produced by systematically removing the sequence’s flanking residues.
These peptide libraries are fully customisable, with modifications and endings to optimise efficiency. Even the direction of truncation can be chosen according to known essential residues.
T-Cell Truncated Peptide Libraries
A version of truncation specific for T-cell epitope mapping, T-Cell Truncated Peptide Libraries test all possible epitopes over the protein of interest.
It is known that peptides of 8-11 residues partially comprise the epitopes of cytotoxic T lymphocytes. With this knowledge, sets of progressively truncated homologous peptides are grouped together for testing. After screening, positive results can be further broken down to identify individual epitopes, saving time and resources.
Epitope Mapping with Peptide Libraries
With Mimotopes’ multipin synthesis system, hundreds of peptides can be produced simultaneously. These high quality peptide libraries are ideal tools for epitope mapping and for the rapid identification of:
- T-Cell clones
- T-Helper Cell clones
- T-Cell lines
- Unpurified T-cells (such as lymph node cells and peripheral blood mononuclear cells, PBMC)