With labels and modifications, changes can be made to a peptide without altering the base amino acid sequence. This versatility allows similar peptides to function in different ways and, in some cases, lead to complete inhibition.
Cyclisation
Cyclisation is an optional modification, to aid in the constrained conformation of a peptide with a disulphide bond. We offer head-to-tail cyclisation, side-chain cyclisation, stapled, and other constrained peptides. Linear peptides, especially those with long sequences, are particularly unpredictable and susceptible to unintentional folding. Cyclised peptides, on the other hand, are more stable.
Bio-conjugation of peptides
Conjugation of a peptide to another molecule, such as an immunogenic carrier protein or oligo has broad applications in immunological and molecular research. Bifunctional pegylation also has applications in pharmacology.
Peptides generally don’t produce an antigenic response on their own, so conjugation facilitates a way to produce anti-peptide antibodies by coupling to a T-cell carrier protein.
Biotinylation
In immunological research and screening applications, capture mechanisms may require biotinylated peptide synthesis. Biotinylation is the addition of a biotin group to the terminus or side chain of a peptide to allow for binding to avidin and streptavidin. This complex is advantageous for the immobilisation of a peptide library, particularly for use in multiple assays.
Phosphorylation
In biological research applications, phosphorylation is a fundamental process. Mimotopes offers phosphopeptide synthesis for cell research, as well as phosphopeptides to be used as antigens in the production of anti-phosphopeptide antibodies.
Methylation, carbamylation, acetylation and glycosylation
Mimotopes offers several optional peptide modifications including carbamylation, glycosylation, methylation and acetylation. These are useful in a wide variety of applications. Peptide methylation, the addition of at least one methyl group, can have applications in epigenetics.
Fluorescent labels and REPLI library
A fluorescent peptide is used in biological experiments to detect a reaction or change. We provide FRET peptides in our REPLI PepSet peptide libraries, fluorescently labelled with an inhibitor for cleavage reactions so that there will be visible fluorescence when cleavage has occurred.
Stable isotope-labelled peptides
Isotope-labelled peptides are often indistinguishable from unlabelled peptides, except under specific conditions. This flexibility allows use in a range of applications, including as a reference in mass spectrometry.
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